|Title||HPLC-ASSAY OF RIFAMPICIN IN HUMAN PLASMA: APPLICATION FOR PROTEIN BINDING STUDY|
Rahul Kumar , Devendra Kumar, Neerja Trivedi Rajbir Singh3 & Rakesh Kumar Dixit
King George’s Medical University, Lucknow, India & NDRI, Karnal, India
A rapid and sensitive HPLC-PDA method was developed for the quantiﬁcation of rifempicin in human plasma using chlorzoxazone as internal standard. The analyte and internal standard were extracted from the plasma sample using a liquid-liquid extraction method. Chromatographic separation was achieved on a Thermo (250 × 4.6 mm, 5 µm) column with a mobile phase consisting 2mM sodium acetate buffer and acetonitrile (50:50, v/v with ); at a flow rate of 1.0 ml /min. The assay was validated with a linear range of 2.5 – 40 µg /ml for rifampicin using 200 µL of plasma sample. The intra- and inter-day assay precision ranged from 1.20% to 3.90% and 0.30to 4.2%, respectively, and intra- and inter- day assay accuracy was between from 0.2 to 4.0% and -0.4% to 2.4%, respectively. The method was successfully applied to the protein binding studies of rifampicin in human plasma.