|Title||NEUROPROTECTIVE EFFECT OF AMLA EXTRACT ON DNA FRAGMENTATION IN CEREBRAL STROKE IN RATS|
Dinesh Tripathi, Sunita Tiwari & Arti Verma
King George’s Medical University, Lucknow, Uttar Pradesh, India
This study was done to determine the neuroprotective effect of amla fruit extract on DNA fragmentation after ischemia reperfusion injury in rats. The MCA of male SD rats were occluded for 60 minutes with the use of an intraluminal technique, and reperfusion was instituted for 24 hours. Amla extract was administered orally 1 hr prior and 3 hours post to ischemia reperfusion injury.DNA fragmentation was measured by performing terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. With amla extract treatment, the number of TUNEL positive cells in the ipsilateral cerebral cortex and striatum region was significantly reduced at 24 hours after reperfusion. In summary, our data suggest that inhibition of apoptosis with amla extract significantly reduces the TUNEL positive cell probably through its antioxidant and anti-inflammatory property.